Movie SM8

Two MTs are bound via Cin8 cluster located at their ends.
Red MTs are immobilized to the glass surface, yellow MTs are free in solution; Cin8 is shown in white. Left panel: immobilized and free MTs and Cin8; right panel: immobilized MTs and Cin8. Two MTs are attached via a Cin8 cluster located at their end while the free MT is pivoting around this cluster. Bar: 10 μm. Time interval between frames: 3.6 s. Movie sped up 36X, 10 fps.

A potential physiological role for bi-directional motility and motor clustering of mitotic kinesin-5 Cin8 in yeast mitosis

Ofer Shapira, Alina Goldstein, Jawdat Al-Bassam, and Larisa Gheber

J Cell Sci 2017. 130:725-734; doi: 10.1242/jcs.195040