Movie SM9

Cin8 crosslinks and slides apart dynamic MTs.
Three-color MT dynamic experiment in the presence of Cin8. Top: GMPCPP-stabilized MTs; middle: dynamic MTs; bottom: Cin8. Top panel: two GMPCPP MTs undergo antiparallel sliding; at the ends of both MTs MT dynamics is observed (middle panel). Note the gap between the two GMPCPP-stable MTs indicated by yellow arrowhead, indicating that these MTs are crosslinked by Cin8 binding to dynamic MTs. Bar: 2 μm; time intervals between frames 3.3 s; Movie sped up 30X, 9 fps.

A potential physiological role for bi-directional motility and motor clustering of mitotic kinesin-5 Cin8 in yeast mitosis

Ofer Shapira, Alina Goldstein, Jawdat Al-Bassam, and Larisa Gheber

J Cell Sci 2017. 130:725-734; doi: 10.1242/jcs.195040