Movie 1

Dynamics of GFP-GRAF1 and mCherry-Rab8 at cell protrusion. Double Flp-In T-REx HeLa cells expressing GFP-GRAF1 (green) and mCherry-Rab8 (red). White arrows and numbers highlight one example per category. Note that several events per category appear throughout the movie. Images were acquired with a spinning disk confocal microscope every 3 seconds for 10 minutes (Zeiss Cell Observer).