Movie 2

Effect of osmotic changes on the localization of GFP-GRAF1 and mCherry-Rab8 in double FLPin cells. Double Flp-In T-REx HeLa cells expressing GFP-GRAF1 (green) and mCherry-Rab8 (red) seeded on microfluidic plates. Normal medium was exchanged to hypotonic medium and replaced 10 minutes later with isotonic medium as indicated. Images were acquired every 3 seconds for 23 minutes with a spinning disk confocal microscope (Zeiss Cell Observer).