Movie 5

(Related to Figure 3). Time-lapse confocal imaging of a X. laevis embryo at NF stage 10-11 undergoing cytokinesis. The embryo was co-injected with MO+MgcSxNN and probes for active RhoA (GFP-rGBD, green) and F-actin (mChe-UtrCH. red). The embryo forms multiple cleavage furrows that ingress to initially divide the cell into thirds. The furrow then regresses, and the embryo ultimately fails cytokinesis. The movie is 16 min long, showing 60 frames with a time interval of 16 s (13 z-planes per time point), played back at 10 fps. Scale = 20 µm.