Movie 8

(Related to Figure 5): Time-lapse confocal imaging of a X. laevis embryo at NF stage 10-11 showing RhoA-GTP (GFP-rGBD) accumulation in dividing Xenopus embryos. Embryos were co-injected with MO+MgcWT (left) or MO+MgcSxNN (right) along with probes for RhoAGTP (GFP-rGBD, shown in greyscale) and F-actin (mChe-UtrCH, not shown). White arrows point to GFP-rGBD accumulation at the equatorial cortex during anaphase onset. The movie is 13 min long, showing 50 frames with a time interval of 16 s (13 z-planes per time point), played back at 10 fps. Scale = 20 µm.