Measuring myosin turnover after purse string ablation. Epidermal cells expressing MRLC:GFP (left, green; centre) and E-cadherin:mTomato (left, magenta; right) in a wounded embryo. White arrowheads indicate photobleaching sites adjacent (top) or far (bottom) from the ablation site. Images were acquired by time-lapse confocal microscopy using a spinning disk confocal microscope (Revolution XD; Andor Technology). A stack was acquired every 4 s for 1 minute. Time is with respect to purse string ablation. Anterior left, dorsal up.