Movie 3

Myosin localization after Y-27632 injection. Wounded embryos expressing wild-type (Sqh-TS, left), unphosphorylatable (Sqh-AA, centre), or phosphomimetic (Sqh-AE, right) myosin. Images were acquired by time-lapse confocal microscopy using a spinning disk confocal microscope (Revolution XD; Andor Technology). A stack was acquired every 30 s for 3 minutes. Time is with respect to Y-27632 injection. Anterior left, dorsal up.