Movie S8
Mroh1 associates with and is removed from the PL by F-actin in fam21 KO cells
Fam21 KO cells co-expressing RFP-Lifeact (left) and Mroh1-GFP (right) imaged by timelapse confocal microscopy. The Mroh1-GFP is clearly associated with the fountains of F-actin streaming off of the PL. These have the effect of stripping the Mroh1 away from the PL and so reducing its concentration there. Scale bar = 10µm.
Mroh1, a lysosomal regulator localized by WASH-generated actin
J Cell Sci 2017. 130:1785-1795; doi: 10.1242/jcs.197210