Movie S9

Mroh1 localization and distribution requires F-actin
Cells expressing Mroh1-GFP treated with 5┬ÁM LatA (at time zero) and imaged by confocal timelapse microscopy. Within 12sec there is a dramatic condensation of Mroh1 onto cytosolic particles and lysosomes. As LatA exposure continues the Mroh1-GFP signal begins to resolve into intense foci on the lysosomes; this transition can take up to 30min to complete.

Mroh1, a lysosomal regulator localized by WASH-generated actin

Peter A. Thomason, Jason S. King, and Robert H. Insall

J Cell Sci 2017. 130:1785-1795; doi: 10.1242/jcs.197210