Movie 2
CAMSAP3-GFP and Tubulin-RFP leaves the centrosome together.
DLD1 cells were transfected with CAMSAP3-GFP and Tubulin-RFP and then went through microtubule regrowth assay. Live-cell imaging was conducted in 1 min intervals after nocodazole washout using a Delta Vision microscope through a 100×oil-immersion lens. Scale bar: 2 μm.
CAMSAP3 accumulates in the pericentrosomal area and accompanies microtubule release from the centrosome via katanin
J Cell Sci 2017. 130:1709-1715; doi: 10.1242/jcs.198010