Movie 1

Readthrough occurs in cytoplasmic foci containing UPF1 but devoid of DCP1a. The experimental procedure is described in the legend of Figure 8. The cellular localization of YFP-UPF1 is in white/green, that of RFP-DCP1a is in white/red and that of GPx1-Neptune is in white/blue. Blue arrows indicate positions of newly synthesized readthrough GPx1-Neptune, green arrows indicate UPF1-containing cytoplasmic foci, and red arrows show DCP1a-containing cytoplasmic foci (P-bodies).