Movie 3
Readthrough does not occur when translation is inhibited. The experiment was performed as for Video S1 except for cells were treated with cycloheximide for 4 hours before to initiate the transcription of Neptune-GPx1 Ter RNA by doxycycline.
Premature termination codon readthrough in human cells occurs in novel cytoplasmic foci and requires UPF proteins
J Cell Sci 2017. 130:3009-3022; doi: 10.1242/jcs.198176