Movie 1

pH fluctuations upon network hyperactivity
Neurons were imaged by time-lapse fluorescence microscopy at λex = 405 nm (1 image/2.58 s, total time: 1320 s, movie played at 29 frames/s) under basal conditions and after administration of bicuculline (30 µM) and TTX (300 nM).

Neuronal hyperactivity causes Na+/H+ exchanger-induced extracellular acidification at active synapses

Martina Chiacchiaretta, Shahrzad Latifi, Mattia Bramini, Manuela Fadda, Anna Fassio, Fabio Benfenati, and Fabrizia Cesca

J Cell Sci 2017. 130:1435-1449; doi: 10.1242/jcs.198564