Supplementary Movie 2

Tracking of CD3ζ-bearing vesicles at the IS in control Jurkat T cells. Control Jurkat T cells were transfected with CD3ζ-mCherry and allowed to settle on anti-CD3/CD28 coated surfaces and recorded under TIRFm. Images were taken each 100 ms for 30 s (video mounted at 10 fps). Imaris tracking analysis and fluorescence images are shown.