mEos-EB3 comet tracking in a HeLa cell. HeLa cells treated with scrambled shRNA were transfected to express mEos-EB3. End-point tracking was performed at 37°C using an inverted Olympus IX71 microscope (Olympus, Hamburg, Germany) and quantified with the ImageJ manual tracking tool (Schindelin et al., 2015).
Alexander Becher, Tim Eiseler, Marc Porzner, Paul Walther, René Keil, Susanne Bobrovich, Mechthild Hatzfeld, and Thomas Seufferlein
J Cell Sci
2017. 130:3374-3387; doi: 10.1242/jcs.200170