Movie S3

Movement of CgA vesicles in a BON cell. Cells were treated with scrambled shRNA and transfected to transiently express CgA-GFP. Cargo retention was induced at 18°C and re-release initiated at permissive temperature. Live imaging was carried out at 37°C using a confocal laser-scanning microscope and vesicles (seen as bright rounded structures) were tracked in several cells within the cytoplasmic area using the ImageJ manual Tracking tool (Schindelin et al., 2015).