Movie S4

Erratical movement of CgA vesicles in a p0071 depleted BON cell. Cells were treated with p0071-directed shRNA and transfected to transiently express CgA-GFP. Cargo retention was induced at 18°C and re-release initiated at permissive temperature. Live imaging was carried out at 37°C using a confocal laser-scanning microscope and intracellularly accumulated CgA vesicles were tracked in several cells within the cytoplasmic area using the ImageJ manual Tracking tool (Schindelin et al., 2015).

The armadillo protein p0071 controls KIF3 motor transport

Alexander Becher, Tim Eiseler, Marc Porzner, Paul Walther, René Keil, Susanne Bobrovich, Mechthild Hatzfeld, and Thomas Seufferlein

J Cell Sci 2017. 130:3374-3387; doi: 10.1242/jcs.200170