Movie S2

Motility of internalized 260 nm microspheres. A live-cell movie showing motile 260 nm microspheres (green) internalized into endolysosomal vesicles (magenta). A cylindrical lens is used to induce ellipticity in the point spread function. The scale bar is 1 μm. The movie was collected at a frame rate of (10 Hz) (or 100 ms/frame) and sped up to 60 frames per second for display purposes.