Supplementary Movie 2
Time lapse of Ire1-mCherry clustering following 2mM DTT treatment over two hours in iron repletion (Low iron SD + 7μm FeCl3 +2mM DTT) or iron depletion conditions (Low iron SD +2mM DTT) and their control (Low iron SD + 3.5μm FeCl3 +2mM DTT) (iron concentrations identical to regular SD).
Iron affects Ire1 clustering propensity and the amplitude of endoplasmic reticulum stress signaling
J Cell Sci 2017. 130:3222-3233; doi: 10.1242/jcs.201715