Movie 3

Related to Fig. 2B. Live-cell movie of RAW264 cells expressing mCitrine-Rac1Q61L.
Time-lapse images of phase-contrast (left) and fluorescence microscopy of mCitrine-Rac1Q61L were acquired with 15-s intervals after IgG-Es addition. The time elapsed after the first frame is shown (min:s). While lamellipodia were formed around the sites of IgG-E binding, typical phagocytic cup formation was not observed. Scale bar: 10 μm.

Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation

Yuka Ikeda, Katsuhisa Kawai, Akira Ikawa, Kyoko Kawamoto, Youhei Egami, and Nobukazu Araki

J Cell Sci 2017. 130:2530-2540; doi: 10.1242/jcs.201749