Related to Fig. 5A. Time-lapse movie of optogenetic control of the PA-Rac1 switch between ON and OFF in RAW264 cells fed with IgG-Es.
RAW264 cells expressing mCherry-PA-Rac1 were photoactivated with ~430 nm light illumination to turn the Rac1 switch ON during feeding with IgG-Es. Subsequently, PA-Rac1 was deactivated by turning off the blue light. The time elapsed after PA-Rac1 ON or OFF is shown in the bottom (min:s). Bar: 10 μm.