Movie 6

Related to Fig. 5B. Time-lapse movie of repeated optogenetic control of PA-Rac1 between ON and OFF.
Rac1 switching between ON and OFF was repeatedly performed during feeding with IgG-Es under an automated optogenetic microscope. Left: phase-contrast images. Right: deconvolution-processed mCherry-PA-Rac1 fluorescence images were displayed as inverted grayscale images to increase the perceptual contrast. The time elapsed after the first frame is indicated in the bottom (min:s). Scale bar: 10 μm.

Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation

Yuka Ikeda, Katsuhisa Kawai, Akira Ikawa, Kyoko Kawamoto, Youhei Egami, and Nobukazu Araki

J Cell Sci 2017. 130:2530-2540; doi: 10.1242/jcs.201749