Related to Fig. 5B. Time-lapse movie of repeated optogenetic control of PA-Rac1 between ON and OFF.
Rac1 switching between ON and OFF was repeatedly performed during feeding with IgG-Es under an automated optogenetic microscope. Left: phase-contrast images. Right: deconvolution-processed mCherry-PA-Rac1 fluorescence images were displayed as inverted grayscale images to increase the perceptual contrast. The time elapsed after the first frame is indicated in the bottom (min:s). Scale bar: 10 μm.