Movie 7

Related to Fig. 6A. Time-lapse movie of Rac1 FRET efficiency showing Rac1 activity levels in live RAW264 cells during phagocytosis of IgG-Es.
RAW264 cells expressing RaichuEV-Rac1, a FRET biosensor for Rac1 activity, were fed with IgG-Es. FRET efficiency representing Rac1 activity is shown in spectrum color (left). The FRET efficiency image (shown in red) is overlaid onto the corresponding phase-contrast images (right). FRET efficiency images were created by the image arithmetic of the FRET/CFP ratio using MetaMorph software. The time elapsed from the first frame is indicated in the frame (min:s). Scale bar: 10 μm.