Movie 9

Related to Fig. 6B. Magnified time-lapse movie of Rac1 FRET efficiency showing Rac1 activity levels during phagocytosis of IgG-coated latex beads.
RAW264 cells expressing RaichuEV-Rac1, a FRET biosensor for Rac1 activity, were fed with IgG-coated latex beads. FRET efficiency representing Rac1 activity is shown in spectrum color (left). The FRET efficiency image (shown in red) is overlaid onto corresponding phase-contrast images (right).Two scenes from the phagocytosis of IgG-coated latex beads are shown. The transient passage of Rac1 activity from the bottom to the top of a phagocytic cup can be seen. The time elapsed from the first frame is indicated in the frame (min:s). Scale bar: 5 μm.