Bidirectional axonal transport vesicles containing TAG-1-GFP and Caspr2-mcherry.
A neuron co-transfected at DIV7 with TAG-1-GFP and Caspr2-mcherry. Axonal transport visualized 16 h after transfection using two-channel overlay recording. Streamed time-lapse images were acquired with 40 frames per 60 s for 220 s. Movie is played at 5.3 frames per second.