Movie 4

Analysis of focal adhesion dynamics in wild type U2OS cells using TIRF microscopy. Wild type U2OS cells were transfected with RFP-zyxin (red) and Emerald-LifeAct (green) to measure changes in focal adhesions over a time course of 25 minutes. Images were acquired continuously every 60 seconds for 25 minutes. Representative focal adhesion points are indicated by arrows. Scales bar indicates 20 μm.