Movie 3

A representative example is shown in which sister CENs interact with a single MT after their cohesion is lost (associated with Figure 2B). The procedure of the experiment is explained in the legend for Figure 2B. Green shows the spindle and MTs while red shows CEN on chromosome XV. The interval of frames is 10 sec, and 5 frames are shown per second in the movie.

Mechanisms mitigating problems associated with multiple kinetochores on one microtubule in early mitosis

Zuojun Yue, Shinya Komoto, Marek Gierlinski, Debora Pasquali, Etsushi Kitamura, and Tomoyuki U. Tanaka

J Cell Sci 2017. 130:2266-2276; doi: 10.1242/jcs.203000