Movie 4

A representative example of a Kar3-depleted cell in which a laterally attached CEN showed detachment after coming into contact with an end-on attached CEN (associated with Figure 3A). The procedure of the experiment is explained in the legend for Figure 3A. Green shows the spindle, MTs and CEN on chromosome III while red shows CEN on chromosome XV. The interval of frames is 18 sec, and 5 frames are shown per second in the movie.