Movie 5

A representative example of CEN detachment is shown in physiological condition (associated with Figure 4A). The procedure of the experiment is explained in the legend for Figure 4A. Green shows the spindle, MTs and CEN5 while red shows KTs. The interval of frames is 10 sec, and 5 frames are shown per second in the movie. Note that, after showing detachment at 210 s, CEN5 was out of focus during 230-310 s, during which CEN5 distance from a spindle pole was measured using out-of-focus CEN5 signals.