Movie 2

Microtubule growth analysis.
MT dynamics were analyzed from EB1::GFP time lapse recordings, using TrackMate. The EB1::GFP recordings were deconvolved with a small S/N ratio to remove background and to enhance signal. Following that, the EB1::GFP signal was further enhanced by a custom made Difference of Gaussian filter. After that, EB1::GFP tracks were analyzed separately. To reduce the ratio of false trajectories all the tracks that could not be followed for at least four consecutive frames were discarded. For the spot detection, we used the LoG detector and the simple LAP tracker were used to link the spots. Gap closing was allowed for one frame gaps.