Movie 4
Effect of latrunculin A (200 nM) on the actin cytoskeleton of neurons expressing Actin5C::GFP.
Actin cytoskeleton starts to breakdown as early as 30-60 seconds after treatment and the stereotypic searching movements of filopodia completely disappear after ~5 minutes.
The formin DAAM is required for coordination of the actin and microtubule cytoskeleton in axonal growth cones
J Cell Sci 2017. 130:2506-2519; doi: 10.1242/jcs.203455