Movie 1

Moonshine mutants are devoid of microglia.
In vivo dorsal view of the midbrain of mon^NQ039Tg(mpeg:mCherry) sibling (left) and mutant (right) larvae at 3 dpf. Macrophages expressing mCherry appear in red fluorescence, neural and other tissues are counter-stained in green with fluorescent Bodipy ceramide (green). As the movies proceed deeper along the Z axis of a single time-point, numerous macrophages can be seen between and around neurons of the optic tectum of the sibling (left), but not in the mutant (right). Orientation, anterior to the left. 20x objective, zoom 3. Sibling, 74 μm z-stack (37 planes, every 2 μm); mutant, 64 μm z-stack (32 planes, every 2 μm).