Movie 3

(related to Fig. 3). Moonshine macrophages do not properly colonize the retina. In vivo lateral time-lapse imaging of the right eye (anterior to the left, dorsal down) of monNQ039Tg(Pu.1:GFP) sibling (left) and mutant (right) embryos at 48 hpf for 12 hrs, every 2.5 min. Myeloid cells expressing GFP were tracked in 3D over time inside (pink dots and tracks) and outside (blue dots and tracks) the eye. The tracks are show at any time point for the last 12 positions (i.e. 30 min.) of the cell. In the sibling, macrophages are present in the retinal tissue as well as moving into and out of it, while in the mutant, few are seen, they mostly stay outside the retina along its vitreal face, and when they occasionally enter the retina, do not stay there (pink tracks). Myeloid cells moving in the mesenchyme around the eye (cyan tracks) are also less numerous and mobile in the mutant (blue tracks). 20× objective, zoom 2×; sibling, 115 μm z-stack (23 planes, every 5 μm); mutant, 125 μm zstack (25 planes, every 5 μm).

Trim33 is essential for macrophage and neutrophil mobilization to developmental or inflammatory cues

Doris Lou Demy, Muriel Tauzin, Mylène Lancino, Véronique Le Cabec, Michael Redd, Emi Murayama, Isabelle Maridonneau-Parini, Nikolaus Trede, and Philippe Herbomel

J Cell Sci 2017. 130:2797-2807; doi: 10.1242/jcs.203471