(related to Fig. 4). Wandering myeloid cells outside the CNS also display migration defects in the moonshine mutant. In vivo confocal time lapse imaging of the cephalic region shown in Fig. 4a,b in 2 days old monNQ039Tg(mpeg1:mCherry;mpx:GFP) sibling (left) and mutant (right). Sibling macrophages (red) and neutrophils (green) in the cephalic mesenchyme around the ear migrate faster and cover greater areas than mutant ones. 20× objective, zoom 1.25×. Timelapse 15 hrs, every 3 min. Sibling, 200 μm z-stack (40 planes spaced by 5 μm); mutant, 140 μm z-stack (28 planes × 5 μm).
Doris Lou Demy, Muriel Tauzin, Mylène Lancino, Véronique Le Cabec, Michael Redd, Emi Murayama, Isabelle Maridonneau-Parini, Nikolaus Trede, and Philippe Herbomel
J Cell Sci
2017. 130:2797-2807; doi: 10.1242/jcs.203471