Movie 4

(related to Fig. 4). Wandering myeloid cells outside the CNS also display migration defects in the moonshine mutant. In vivo confocal time lapse imaging of the cephalic region shown in Fig. 4a,b in 2 days old mon^NQ039Tg(mpeg1:mCherry;mpx:GFP) sibling (left) and mutant (right). Sibling macrophages (red) and neutrophils (green) in the cephalic mesenchyme around the ear migrate faster and cover greater areas than mutant ones. 20× objective, zoom 1.25×. Timelapse 15 hrs, every 3 min. Sibling, 200 μm z-stack (40 planes spaced by 5 μm); mutant, 140 μm z-stack (28 planes × 5 μm).