Movie 5

(related to Fig. 5). Wounding does not recruit myeloid cells in the moonshine mutant. Time-lapse imaging and cell tracking of macrophages and neutrophils during the first 3hrs following transection of the tail fin tip at 3 dpf in mon^NQ039Tg(mpeg1:mCherryF;mpx:GFP) sibling (left) and mutant (right) larvae. The tail fin wound induces a fast and efficient (track straightness and cell number) recruitment of both macrophages (red tracks) and neutrophils (green tracks) in the sibling, but not in the moonshine mutant. Orientation: dorsal to the left, caudal down. Time-lapse duration 200 min, every 7 min. 20× objective, zoom 1×; sibling, z-stack 185 μm (37 planes × 5 μm); mutant, z-stack 190 μm (38 planes × 5 μm).