Movie 6

(related to Fig. 5D-F and Fig. S5). LTB4 bath does not induce neutrophil migration to the fin and skin in moonshine mutants. Confocal imaging (anterior to the left, dorsal up) of the CHT region of monNQ039Tg(mpeg1:mCherryF;mpx:GFP) sibling (left) and mutant (right) larvae. LTB4 was added to the embryo water at t13 of the parallel timelapse imaging (3,75 hrs, every 6 min) of the two larvae. It induced a fast and massive relocalization of neutrophils in the skin and fin of the sibling embryo, but not in the mutant. 20x objective, zoom 1×. Sibling, z-stack 145 μm (29 planes × 5 μm); mutant, z-stack 240 μm (48 planes × 5 μm).

Trim33 is essential for macrophage and neutrophil mobilization to developmental or inflammatory cues

Doris Lou Demy, Muriel Tauzin, Mylène Lancino, Véronique Le Cabec, Michael Redd, Emi Murayama, Isabelle Maridonneau-Parini, Nikolaus Trede, and Philippe Herbomel

J Cell Sci 2017. 130:2797-2807; doi: 10.1242/jcs.203471