Movie 7

(related to Fig. 6A-H). Gram-negative bacteria infection does not recruit myeloid cells in the moonshine mutant. Time-lapse confocal imaging (7 hrs, every 6.5 min; lateral view, anterior to the left) of mon^NQ039Tg(mpeg1:mCherryF;mpx:GFP) sibling (left) and mutant (right) embryos at 60 hpf, whose left ear was injected with CFP+ (channel not shown) E. Coli 20 min. earlier. Bacteria in the ear cavity induce a fast and efficient recruitment of both macrophages (red cells) and neutrophils (green cells) in the sibling, but not in the moonshine mutant. 20× objective, zoom 1×; 235 μm z-stacks (47 planes × 5μm).