Movie 8

(related to Fig. 6I,J). Gram-positive bacteria infection does not recruit myeloid cells in the moonshine mutant. Time-lapse confocal imaging (6 hrs, every 2.5 min.) of 72 hpf mon^NQ039Tg(LysC:DsRed) sibling (left) and mutant (right), following injection of GFP+ B.subtilis bacteria in the ear cavity 20 min. earlier. Bacteria in the ear cavity induce a fast and efficient recruitment of neutrophils (red cells) in the sibling, but not in the moonshine mutant. Orientation, anterior to the left. 20× objective, zoom 1×. Sibling: 125 μm z-stack (25 planes spaced by 5 μm); mutant: 130 μm z-stack (26 planes).