Movie 9

(related to Fig. 7). The basal interstitial migration defect of neutrophils in moonshine mutants is cell-autonomous. Parallel time-lapse imaging (3hrs, every 2.5 min) of the ear region of 2 days-old Tg(mpx:GFP) wild-type (left) and moonNQ039Tg(gata1:DsRed;LysC:DsRed) mutant (right) parabiotic embryos. Note that wild-type (green) and mutant (red) neutrophils have colonized both parabiotes, allowing to track their migratory behaviour in both wt and mutant environment. Orientation, anterior up. 20× objective, zoom 1×. WT, z-stack 145 μm (29 planes × 5 μm); mutant, z-stack 130 μm (26 planes × 5um).

Trim33 is essential for macrophage and neutrophil mobilization to developmental or inflammatory cues

Doris Lou Demy, Muriel Tauzin, Mylène Lancino, Véronique Le Cabec, Michael Redd, Emi Murayama, Isabelle Maridonneau-Parini, Nikolaus Trede, and Philippe Herbomel

J Cell Sci 2017. 130:2797-2807; doi: 10.1242/jcs.203471