Normal spindle formation and mitotic progression after ASPM KO
SiR-tubulin images were acquired every 3 min with spinning-disc confocal microscopy. Images were acquired with 5 z-sections (separated by 3 μm) and displayed after maximum projection. Time 0 corresponds to the timing of nuclear envelope breakdown. The images of two KOExon1 lines (#4 and #23) and two complete KOC lines (#25 and #27) are presented.