CDK5RAP2 degradation impairs spindle pole focusing and mitotic progression in the absence of ASPM
Images were acquired every 3 min with spinning-disc confocal microscopy. Green, CDK5RAP2-mAID-mCherry (control cell only); magenta, Sir-Tubulin. Images were acquired with 5 z-sections (separated by 3 μm) and displayed after maximum projection. Time 0 corresponds to the timing of nuclear envelope breakdown.