Movie 3

Microcephaly mutation of ASPM impairs spindle focusing and mitotic progression in the absence of CDK5RAP2
Images were acquired every 5 min with spinning-disc confocal microscopy. Yellow, CDK5RAP2-mAID-mCherry; magenta, Sir-Tubulin; cyan, ASPM-mClover (full-length or C-terminal truncation that mimics microcephaly mutations). Images were acquired with 5 z-sections (separated by 3 μm) and displayed after maximum projection. Time 0 corresponds to the timing of nuclear envelope breakdown.

Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2

Elsa A. Tungadi, Ami Ito, Tomomi Kiyomitsu, and Gohta Goshima

J Cell Sci 2017. 130:3676-3684; doi: 10.1242/jcs.203703