Microcephaly mutation of ASPM impairs spindle focusing and mitotic progression in the absence of CDK5RAP2
Images were acquired every 5 min with spinning-disc confocal microscopy. Yellow, CDK5RAP2-mAID-mCherry; magenta, Sir-Tubulin; cyan, ASPM-mClover (full-length or C-terminal truncation that mimics microcephaly mutations). Images were acquired with 5 z-sections (separated by 3 μm) and displayed after maximum projection. Time 0 corresponds to the timing of nuclear envelope breakdown.