Supplemental Movie 2

Mitochondrial dynamics are impaired upon EHD1-depletion. Live imaging was performed on RPE cells incubated with Mitotracker Red and either Mock-treated (Supplemental Movie 1) or treated with EHD1-siRNA for 72 h (Supplemental Movie 2). 4-slice Z-section images were taken every 15 sec for 5 min for each treatment, and compiled to a maximal projection image.