To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Supplemental Movie 4 KG1a cells migrating upstream on an ICAM-1 surface at a shear rate of 800s−1 with isotype blocking antibody Migration against the direction of flow is LFA-1-dependent in human hematopoietic stem and progenitor cells Alexander Buffone, Nicholas R. Anderson, and Daniel A. Hammer J Cell Sci 2018. 131:None-None; doi: 10.1242/jcs.205575 Download Original Video