Movie 2

(A) Inverted movie shows clathrin dynamics at the ventral surface of a BSC-1 cell at different levels of squeezing. (B) Clathrin coat growth rate distributions are assembled for each time point of the movie. (C) Growth rate distributions are shown as bar plots, where each bin represents a different growth phase (FD, fast dissolution; SD, slow dissolution; P, plateau; SF, slow formation; FF, fast formation)(Ferguson et al., 2016). Squeezing steps can be observed as stepwise changes in the growth phases. Note that the high magnitude growth rates (FD & FF) diminish as the P phase gets more occupied with the increasing membrane tension.