Movie 4

Movie shows clathrin coats at the ventral surface of a migrating astrocyte. The lamella extends towards the top-right corner of the image. Plots show the clathrin coat growth rate distributions, the SD of growth rate distributions, the area of the cell, average clathrin coat lifetime and initiation density of clathrin-coated structures. The arrow appearing at t = 177s marks the poking of the lamella by a micromanipulator controlled glass tip. The lamella starts to retract subsequently (see the change in the cell area). Note the increased number/density of clathrin-coated structures associated with the retraction.