To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Supplemental Movie 3 Representative movies of cells expressing iLID-Caax (green) in combination with Tiam-Micro (red). Photoactivation occurs in the ROI highlighted with a white box. Cells are plated on either fibronectin or poly-L-lysine coated cover glass. Cells lay their own tracks – optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration Seth P. Zimmerman, Sreeja B. Asokan, Brian Kuhlman, and James E. Bear J Cell Sci 2017. 130:2971-2983; doi: 10.1242/jcs.205948 Download Original Video