To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Supplemental Movie 4 Representative movie of cells expressing Halo-iLID-Caax in combination with ITSN-Micro (red) and Venus-Paxillin (green). Photoactivation occurs in the ROI highlighted with a white box. Cells are plated on poly-L-lysine coated cover glass. Cells lay their own tracks – optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration Seth P. Zimmerman, Sreeja B. Asokan, Brian Kuhlman, and James E. Bear J Cell Sci 2017. 130:2971-2983; doi: 10.1242/jcs.205948 Download Original Video