Nocodazole effects on macrophages migrating to a wound. When migrating to a wound (in the presence of nocodazole) macrophages show rear detachment phenotype (see arrow). Front edge of the cell keeps migrating to the wound, while rear end stays attached. No neutrophils showed this phenotype. Upper panel: macrophage and neutrophils only, lower panel: macrophage, neutrophils and brighfield. Scale bar, 100μm